PANOPLY met à la disposition de la communauté scientifique académique et industrielle l'éventail des ressources de hautes technologies du Plateau de Saclay dans le domaine des sciences de la Terre, du Climat et de l’environnement. https://panoply-geops.lsce.ipsl.fr/index.php/en/itemid-fix2-english-version 2026-06-19T16:53:58+00:00 Panoply nada.caud@lsce.ipsl.fr Joomla! - Open Source Content Management 2020-10-23T05:53:16+00:00 2020-10-23T05:53:16+00:00 https://panoply-geops.lsce.ipsl.fr/index.php/en/itemid-fix2-english-version/295-fiche-52-gc-prep-2 Jacob jeremy.jacob@lsce.ipsl.fr

<p style="text-align: center;"><strong><span style="font-family: arial, helvetica, sans-serif; font-size: 14pt;">Fraction collector (Gerstel Preparative Fraction Collector) coupled to a gas chromatograph (Agilent 6890 N) and an autosampler (Gerstel MPS 2)<br /></span></strong></p> <p><img src="https://panoply-geops.lsce.ipsl.fr/images/IMG_20210428_184008-768x576.jpg" alt="IMG 20210428 184008 768x576" width="571" height="428" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt;"><strong><span style="color: #000000;">Instrument description:</span><br /></strong></span></p> <p><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt; color: #000000;">Autosampler (Gerstel MPS 2): The autosampler takes a few µL of the fraction in solution in an organic solvent and introduces it into the gas chromatograph injector.</span></p> <p><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt; color: #000000;">Gas chromatograph (Agilent 6890 N): Molecular biomarkers are separated by molecular weight and polarity in a capillary column under helium flow, as the chromatography oven heats up.</span></p> <p><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt; color: #000000;">Fraction collector (Gerstel Preparative Fraction Collector): at the end of the capillary column, molecular biomarkers are collected in liquid nitrogen-cooled tubes. 7 fractions can be collected.</span></p> <p><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt; color: #000000;">The system is used to purify, separate and collect molecular biomarkers (lipids, sugars, amino acids, etc.) extracted from geological and archaeological matrices (soils, sediments, peat, ceramics etc.), and then determine their age by carbon-14 dating. Extracted molecular biomarkers may first be fractionated into chemical families (alkanes, ketones, alcohols, etc.). Depending on the concentration of molecular biomarkers and the quantity of carbon required for dating, the operation may be repeated several dozen times, until sufficient material for ¹⁴C measurement is obtained (at least 10 µgC).</span></p> <p>&nbsp;</p> <p><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt; color: #000000;"><strong>Analyses performed on the instrument:</strong></span></p> <p><span style="font-size: 12pt; font-family: arial, helvetica, sans-serif;"><span style="color: #000000;">Purification, separation and collection of molecular biomarkers</span><br /></span></p> <p><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt;"></span></p> <p><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt;"><strong><span style="color: #000000;">Contacts:</span><br /></strong></span></p> <p><span style="font-size: 12pt; font-family: arial, helvetica, sans-serif;">Caroline Gauthier : <a href="mailto:caroline.gauthier@lsce.ipsl.fr">caroline.gauthier[a]lsce.ipsl.fr</a></span></p> <p><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt;">Emmanuelle Casanova : <a href="mailto:emmanuelle.casanova@lsce.ipsl.fr">emmanuelle.casanova[a]lsce.ipsl.fr</a></span></p> <p><span style="font-size: 12pt; font-family: arial, helvetica, sans-serif;">Jérémy Jacob : <a href="mailto:jeremy.jacob@lsce.ipsl.fr">jeremy.jacob[a]lsce.ipsl.fr</a></span></p> <p><span style="font-size: 12pt; font-family: arial, helvetica, sans-serif;">Christine Hatté : <a href="mailto:christine.hatte@lsce.ipsl.fr">christine.hatte[a]lsce.ipsl.fr</a></span></p>

<p style="text-align: center;"><strong><span style="font-family: arial, helvetica, sans-serif; font-size: 14pt;">Fraction collector (Gerstel Preparative Fraction Collector) coupled to a gas chromatograph (Agilent 6890 N) and an autosampler (Gerstel MPS 2)<br /></span></strong></p> <p><img src="https://panoply-geops.lsce.ipsl.fr/images/IMG_20210428_184008-768x576.jpg" alt="IMG 20210428 184008 768x576" width="571" height="428" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt;"><strong><span style="color: #000000;">Instrument description:</span><br /></strong></span></p> <p><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt; color: #000000;">Autosampler (Gerstel MPS 2): The autosampler takes a few µL of the fraction in solution in an organic solvent and introduces it into the gas chromatograph injector.</span></p> <p><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt; color: #000000;">Gas chromatograph (Agilent 6890 N): Molecular biomarkers are separated by molecular weight and polarity in a capillary column under helium flow, as the chromatography oven heats up.</span></p> <p><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt; color: #000000;">Fraction collector (Gerstel Preparative Fraction Collector): at the end of the capillary column, molecular biomarkers are collected in liquid nitrogen-cooled tubes. 7 fractions can be collected.</span></p> <p><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt; color: #000000;">The system is used to purify, separate and collect molecular biomarkers (lipids, sugars, amino acids, etc.) extracted from geological and archaeological matrices (soils, sediments, peat, ceramics etc.), and then determine their age by carbon-14 dating. Extracted molecular biomarkers may first be fractionated into chemical families (alkanes, ketones, alcohols, etc.). Depending on the concentration of molecular biomarkers and the quantity of carbon required for dating, the operation may be repeated several dozen times, until sufficient material for ¹⁴C measurement is obtained (at least 10 µgC).</span></p> <p>&nbsp;</p> <p><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt; color: #000000;"><strong>Analyses performed on the instrument:</strong></span></p> <p><span style="font-size: 12pt; font-family: arial, helvetica, sans-serif;"><span style="color: #000000;">Purification, separation and collection of molecular biomarkers</span><br /></span></p> <p><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt;"></span></p> <p><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt;"><strong><span style="color: #000000;">Contacts:</span><br /></strong></span></p> <p><span style="font-size: 12pt; font-family: arial, helvetica, sans-serif;">Caroline Gauthier : <a href="mailto:caroline.gauthier@lsce.ipsl.fr">caroline.gauthier[a]lsce.ipsl.fr</a></span></p> <p><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt;">Emmanuelle Casanova : <a href="mailto:emmanuelle.casanova@lsce.ipsl.fr">emmanuelle.casanova[a]lsce.ipsl.fr</a></span></p> <p><span style="font-size: 12pt; font-family: arial, helvetica, sans-serif;">Jérémy Jacob : <a href="mailto:jeremy.jacob@lsce.ipsl.fr">jeremy.jacob[a]lsce.ipsl.fr</a></span></p> <p><span style="font-size: 12pt; font-family: arial, helvetica, sans-serif;">Christine Hatté : <a href="mailto:christine.hatte@lsce.ipsl.fr">christine.hatte[a]lsce.ipsl.fr</a></span></p> 2015-12-15T09:14:52+00:00 2015-12-15T09:14:52+00:00 https://panoply-geops.lsce.ipsl.fr/index.php/en/by-analyses Super User web.extremoscope@lsce.ipsl.fr

<ul> <li> <p><a href="https://panoply-geops.lsce.ipsl.fr/index.php/en/analyses-en/mineralogical">Mineralogical</a></p> </li> <li> <p><a href="https://panoply-geops.lsce.ipsl.fr/index.php/en/analyses-en/elemental">Elemental</a></p> </li> <li> <p><a href="https://panoply-geops.lsce.ipsl.fr/index.php/en/analyses-en/isotopic">Isotopic</a></p> </li> <li> <p><a href="https://panoply-geops.lsce.ipsl.fr/index.php/en/analyses-en/radioactivity-measurement">Radioactivity measurement</a></p> </li> </ul>

<ul> <li> <p><a href="https://panoply-geops.lsce.ipsl.fr/index.php/en/analyses-en/mineralogical">Mineralogical</a></p> </li> <li> <p><a href="https://panoply-geops.lsce.ipsl.fr/index.php/en/analyses-en/elemental">Elemental</a></p> </li> <li> <p><a href="https://panoply-geops.lsce.ipsl.fr/index.php/en/analyses-en/isotopic">Isotopic</a></p> </li> <li> <p><a href="https://panoply-geops.lsce.ipsl.fr/index.php/en/analyses-en/radioactivity-measurement">Radioactivity measurement</a></p> </li> </ul>